solubilization solution Search Results


solubilization solution  (Promega)
90
Promega solubilization solution
Solubilization Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubilization solution - by Bioz Stars, 2026-08
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solubilization solution/stop mix  (Promega)
90
Promega solubilization solution/stop mix
Solubilization Solution/Stop Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubilization solution/stop mix - by Bioz Stars, 2026-08
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mtt solubilizing solution  (Promega)
90
Promega mtt solubilizing solution
Mtt Solubilizing Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubilization solution (dimethyl sulfoxide)  (SERVA Electrophoresis)
90
SERVA Electrophoresis solubilization solution (dimethyl sulfoxide)
Solubilization Solution (Dimethyl Sulfoxide), supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/pmc07250418-158-10-14?v=SERVA+Electrophoresis
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solubilization solution (dimethyl sulfoxide) - by Bioz Stars, 2026-08
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dmso-solubilized peptide solution  (Air-Tite Inc)
90
Air-Tite Inc dmso-solubilized peptide solution
Dmso Solubilized Peptide Solution, supplied by Air-Tite Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmso-solubilized peptide solution - by Bioz Stars, 2026-08
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solubilization solution/stop mix g401a  (Promega)
90
Promega solubilization solution/stop mix g401a
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Solubilization Solution/Stop Mix G401a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/pmc11538497-197-2-23?v=Promega
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solubilization solution/stop mix g401a - by Bioz Stars, 2026-08
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stop solubilization solution  (Promega)
90
Promega stop solubilization solution
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Stop Solubilization Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/us08609627-736-5-8?v=Promega
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stop solubilization solution - by Bioz Stars, 2026-08
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solubilization solution  (EuroClone)
90
EuroClone solubilization solution
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Solubilization Solution, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/pmc04682184-101-10-23?v=EuroClone
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solubilization solution - by Bioz Stars, 2026-08
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tmb-solubilized substrate solution tmb one  (Promega)
90
Promega tmb-solubilized substrate solution tmb one
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Tmb Solubilized Substrate Solution Tmb One, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/pmc10144510-78-12-19?v=Promega
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protein-bound dye solubilized in tris base solution  (Promega)
90
Promega protein-bound dye solubilized in tris base solution
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Protein Bound Dye Solubilized In Tris Base Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/pmc10803019-135-2-12?v=Promega
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tmb-solubilized substrate solution (tmbone ® 3, 3′, 5, 5′, tetramethylbenzidine)  (Promega)
90
Promega tmb-solubilized substrate solution (tmbone ® 3, 3′, 5, 5′, tetramethylbenzidine)
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Tmb Solubilized Substrate Solution (Tmbone ® 3, 3′, 5, 5′, Tetramethylbenzidine), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tmb-solubilized substrate solution (tmbone ® 3, 3′, 5, 5′, tetramethylbenzidine) - by Bioz Stars, 2026-08
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solutions for the extraction and solubilization of gluten  (Biomedal Inc)
90
Biomedal Inc solutions for the extraction and solubilization of gluten
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Solutions For The Extraction And Solubilization Of Gluten, supplied by Biomedal Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


TNF-α and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells (HAOEC). ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .

Journal: Scientific Reports

Article Title: VE-cadherin shedding in vitro and in patients with aortic aneurysm and dissection

doi: 10.1038/s41598-024-77940-3

Figure Lengend Snippet: TNF-α and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells (HAOEC). ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .

Article Snippet: Viability of HAOEC upon treatment with various TNF-α concentrations ranging from 100 to 1000 ng/ml was performed by an commercially available “MTT” assay (Promega, G4000, including Solubilization Solution/Stop Mix G401A and Dye Solution G402A) as described by Guo et al. .

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Diffusion-based Assay